APC
Activated Protein C Resistance (APC-R) Clotting Assay
EPIC Test Procedure Code: LAB1957
Performing Lab:
Saint Luke's Regional Laboratories
Container Type:
Light blue top (sodium citrate) tube
Specimen Type:
Platelet Poor Plasma
(EDTA or sodium citrate cells must also be sent to perform the Factor V Leiden Mutation, if warranted)
Preferred Volume:
2 mL
Minimum Volume:
1 mL
Collection Procedure:
Specimen Processing:
1. Centrifuge specimen in a centrifuge that has been validated to ensure platelet poor plasma.
2. Immediately transfer the plasma to a stoppered, plastic tube and centrifuge again for 10 minutes.
3. Transfer the supernatant plasma into a screw-capped, plastic vial and send frozen at ≤ -20°C, if possible.
4. If unable to centrifuge, forward unprocessed sodium citrate whole blood (minimum volume: 2 mL) to the laboratory promptly at room temperature only.
Store and Transport:
Frozen (plasma)
Stability:
Unspun specimens must arrive within 2 hours of collection.
CPT Codes:
85307 - Activated Protein C Resistance (EAP 30020020)
81241 - FAC VL (if performed)
Test Schedule:
Monday - Friday
Test Includes:
FAC VL "Factor V Leiden Mutation" will be performed and charged to confirm abnormal results.
Reference Ranges:
≥ 2.50