LPAGF
Candida albicans Antigen Response
EPIC Test Procedure Code: LAB3674
Performing Lab:
Referral Laboratory
Container Type:
Green top (sodium heparin) tube
Specimen Type:
Whole blood
Preferred Volume:
8 mL
Minimum Volume:
8 mL
Collection Procedure:
Specimens must be sent to Ref Lab STAT on the day of collection.
Specimen Processing:
Specimens must arrive at the testing laboratory (ViraCor Eurofins) within 24 hours of collection.
Collect specimens Monday through Thursday only, and not the day before a holiday.
Store and Transport:
Room temperature
Stability:
Room temperature - 24 hours
Unacceptable Condition:
1. Lithium heparin specimens will be rejected.
2. Specimens not received at room temperature will be rejected.
3. Specimens greater than 24 hours old when received will be rejected.
CPT Codes:
86353 - Candida Albicans Ag Response (EAP 30021454)
Method:
Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37 degrees C. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, and CD69 and intracellular IFN-gamma), and analyzed by flow cytometry.