RSV

Respiratory Syncytial Virus (RSV) Antigen, Nasopharyngeal Aspirate

Performing Lab:
Saint Luke's Regional Laboratories
Specimen Type:

Submit only 1 of the following specimens:
Nasopharyngeal aspirate, nasopharyngeal swab, nasopharyngeal wash or tracheal aspirate

Collection Procedure:

Follow current PSC procedures for registering patient and preparing test order.

Aspirate/Wash
1. Collect >1 mL of nasopharyngeal aspirate, nasopharyngeal wash, or tracheal aspirate with a Deelee® trap, sputum trap, or a disposable feeding line attached to a 5 mL syringe. (Do not place in viral transport media.)
2. If a feeding line and syringe combination is used, do not cut the hub off the line; keep the complete line intact.
3. Place the aspirate in a screw-capped, sterile vial.
4. Label the vial with the patient’s full name, date of birth, identification number, date and time of collection, initials of the person collecting the specimen, and the specimen source.
5. Send the specimen refrigerated.
6. Maintain sterility and forward promptly.
Note: The specimen source is required on the request form for processing.

Swab 
1. Obtain M-4 viral transport media. 
2. Collect swab using the following CDC (Center for Disease Control and Prevention) guidelines:
a. Tilt patient's head back 70 degrees.
b. Insert swab into nostril.  (Swab should reach a depth equal to distance from nostrils to outer opening of the ear.)
c. Leave swab in place for several seconds to absorb secretions.
d. Slowly remove swab while rotating it.  (Swab both nostrils with same swab.)
e. Place tip of swab into M-4 viral transport media or 0.5 mL to 1 mL of sterile saline and snap/cut off the applicator stick.
3. Label the tube with the patient’s full name, date of birth, identification number, date and time of collection, initials of the person collecting the specimen, and the specimen source.
4. Maintain sterility and forward promptly at room temperature only.
Note: The specimen source is required on the request form for processing.

 

Patient's head shoudl be inclined 70 degrees as shown for proper specimen recovery
Store and Transport:
Refrigerated: aspirate/wash
CPT Codes:

87807 - RSV Detection (EAP 30063020)

Test Schedule:
Monday through Sunday
Method:
With the availability of ribavirin therapy for serious RSV infections, rapid diagnostic tests for this virus have become increasingly important.
Reference Ranges:

Negative

Lab Personnel

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