BCRABL PCR
BCR/ABL, Quant, PCR
EPIC Test Procedure Code: LAB3588
Synonyms:
BCR ABL PCR
Performing Lab:
Referral Laboratory
Container Type:
Lavender top (EDTA), green top (heparin), or yellow top (ACD-A) tube
Specimen Type:
Whole blood or bone marrow. Cell pellet is also acceptable.
Preferred Volume:
3 - 5 mL
Minimum Volume:
1 mL
Specimen Processing:
1. Specimen should arrive at referral laboratory within 48 hrs of collection. If specimen is to be stored for more than 48 hrs, specimen must be refrigerated.
2. Full test name is BCR-ABL1 Transcript Detection for Chronic Myelogenous Leukemia and Acute Lymphocytic Leukemia, Quantitative.
3. This quantitative test is used to monitor the response of patients to imatinib mesylate or other therapies.
4. Indicate the date and time of collection when ordering on the request form.
5. Enter the source in the HLAB prompt.
Store and Transport:
Room temperature
CPT Codes:
81206 - BCR-ABL1 B2A2 Transcript (EAP 30040545)
81207 - BCR-ABL1 B3A2 Transcript (EAP 30040546)
Method:
Total RNA is isolated from the sample and subject to a real-time, reverse transcriptase polymerase chain reaction (RT-PCR). The PCR primers and probes are specific for BCR-ABL1 e13a2, e14a2 and e1a2 fusion transcripts. The ABL1 transcript is amplified as the control for cDNA quantity and quality. Serial dilutions of a validated positive control RNA with known t(9;22) BCR-ABL1 are used as reference for quantification of BCR-ABL1 relative to ABL1. The numeric BCR-ABL1 level is reported as % BCR-ABL1/ABL1 and the detection sensitivity is 4.5 log below the standard baseline (<0.0032%).
Notes:
This test is not available on a tissue block or fixed tissue.