BACT PCR

Broad Range Bacterial PCR and Sequencing

EPIC Test Procedure Code: LAB7123

Synonyms:
Universal PCR Bacterial
Universal PCR AFB
Performing Lab:
Referral Laboratory
Container Type:
Sterile container
Preferred Volume:
Tissue: 5 mm(3) (approximately the size of a pencil eraser)
Body Fluid: 0.5 mL
Collection Procedure:

Submit only 1 of the following specimens:

Fresh Tissue or biopsy:
1. Collect fresh tissue specimen (at least 5 mm(3)-approximately the size of a pencil eraser.
2. Submit tissue only, do not add fluid to tissue.
3. Keep refrigerated. Reference Lab will freeze specimen.

Body Fluid:
1. Collect fresh fluid specimen.
2. Keep refrigerated. Reference Lab will freeze specimen.

CSF:
1. Collect fresh fluid specimen using sterile technique.
2. Submit specimen from collection vial 2 or higher, specimens in vial 1 are not acceptable.
3. Indicate on the label which vial is being submitted.
4. Keep refrigerated. Reference Lab will freeze specimen.

Ref Lab: see ordering instructions specific for CSF!

Synovial Fluid:
1. Send specimen in original tube (preferred). If unable to send original container, aliquot to sterile screw top tube.
2. Keep refrigerated. Reference Lab will freeze specimen.

Specimen Processing:

Do NOT add saline!

Store and Transport:
Keep refrigerated; Reference Lab will freeze
Ref Lab: send frozen
Stability:

Refrigerated: <21 days

Frozen (preferred): <21 days

Unacceptable Condition:

Reject due to:

Specimen received in anaerobe vial
Tissue received in any fluid (saline, broth, formalin, formaldehyde, acetone, etc)
Wrapping (gauze, drapes, etc)
Blood Culture bottles (Bactec FX and/or BacT/ALERT bottles)
Bone marrow
Decalcified bone
Slides
Skin biopsy
Colon biopsy

CPT Codes:

87801 - Bacterial/AFB mult organisms DNA Amplified (EAP: 30633309)

  • Possible reflex testing:
  • 87798 - Bacterial/AFB Ident by Sequencing (EAP: 30633312)
  • 87798 - Bacterial/AFB Ident by NGS (EAP: 30633313)
  • 87798 - Bacterial/AFB Ident by PCR (EAP: 30633314)
Method:
Polymerase Chain Reaction (PCR) followed by Sequencing
Test Includes:
Test includes testing for bacteria and mycobacteria

Lab Personnel

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