JAK2 REFLEX
JAK2 V617F Mutation Detection with Reflex
EPIC Test Procedure Code: LAB5137
Performing Lab:
Referral Laboratory
Container Type:
Lavender top (EDTA) tube or green top (sodium heparin) tube
Specimen Type:
Whole blood OR bone marrow
Preferred Volume:
3 to 5 mL whole blood OR 1 to 2 mL bone marrow
Minimum Volume:
3 mL whole blood OR 1 mL bone marrow
Collection Procedure:
Collect specimens Monday through Thursday, specimen should arrive at LabCorp within 48 hours of collection.
Store and Transport:
Room Temperature
Unacceptable Condition:
Sample greater than 72 hours old; clotted blood.
CPT Codes:
81270 - JAK2 Reflex (EAP 30150759)
Possible reflex tests:
81219 (CALR) (EAP: 30150760)
81279 (JAK2 Exon 12-15) (EAP: 30150761)
81338 (MPL) (EAP: 30150762)
Method:
Allele-specific polymerase chain reaction (PCR); capillary electrophoresis; Sanger sequencing
Test Includes:
The JAK2 V617F test is performed, if negative reflexes to CALR mutation analysis, JAK2 exon 12-15 mutation analysis and MPL mutation analysis.