Referral Laboratory

1. Place infant's limb in a position to increase venous pressure.
2. Warm site with soft cloth, moistened with warm water up to 41 degrees Celsius, for three to five minutes.
3. Cleanse site with alcohol prep. Allow area to AIR DRY.

Complete ALL information on the collection form, use a ballpoint pen, and print.

Kansas Department of Health & Environmental Laboratories Neonatal Screening Card

Blood spot

Fill all circles completely.

1. Puncture heel, avoid previous puncture sites or curvature of the heel. Make puncture using sterile lancet with top no longer than 2.5 mm or use heel-stick device. Wipe away first blood drop with sterile gauze pad. Allow another LARGE blood drop to form.
2. Gently apply filter paper to large drop of blood BUT NOT the heel. Allow blood to soak through and completely fill preprinted circle. Apply blood to one side of filter paper only. DO NOT layer, or apply blood more than once for the same collection circle. DO NOT touch heel to filter paper.
3. Fill remaining circles in the same manner. If blood flow is diminished, puncture a new sample site and repeat.

1. Allow blood spots to thoroughly air dry for at least 3 hours in a horizontal position away from heat and sunlight, preferably on a rack. Avoid touching or smearing blood spots. Never superimpose one filter paper on another before thoroughly drying.
2. Replace the white paper overlay over filter paper after blood spots have dried. Do not tear off or dispose of the paper overlay. Mail the specimen within 24 hours of collection.

Room temperature

Congenital Hypothyroidism, Congenital Adrenal Hyperplasia, Hemoglobinopathy, Biotinidase Deficiency, Galactosemia, Fatty Acid Disorders, Organic Acid Disorders, Amino Acid Disorders, Cystic Fibrosis, Severe Combined Immunodeficiency.