LPMAF
T Cell Fx Complete
EPIC Test Procedure Code: LAB3675
Synonyms:
Lymphocyte Proliferation Mitogen, Antigen Panel
Performing Lab:
Referral Laboratory
Container Type:
Green top (sodium heparin) tube only
Specimen Type:
Whole blood
Preferred Volume:
8 mL
Minimum Volume:
8 mL
Collection Procedure:
Specimens must be received by the Reference Lab same day of collection, and arrive at the testing laboratory (VirCor Eurofins within 24 hours). Collect specimens Monday through Thursday only, and not the day before a holiday.
Store and Transport:
Room temperature
Stability:
Room temperature - 24 hours
Unacceptable Condition:
Causes for rejection: specimen collected in lithium heparin tube, ACD, or EDTA, not received at room temperature, or specimen greater than 24 hours old when received at the testing laboratory.
CPT Codes:
86353x5 - Lymph Stimulation Panel (EAP 30040586)
Method:
Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37 degrees C. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, and CD69 and intracellular IFN-gamma, and analyzed by flow cytometry.