Plasma, serum and whole blood are all required for this test.

Specimen Requirements

Whole Blood for Factor V Leiden and Prothrombin Gene Mutation
Container /Tube:  Lavender top (EDTA) tube or light blue top (sodium citrate) tube
Specimen: 4 mL (minimum volume: 2 mL) of EDTA or sodium citrate whole blood
Transport Temperature: Room temperature
Note: Label the specimen appropriately (whole blood for Factor V Leiden and Prothrombin Gene Mutation).

Serum for Beta-2 Glycoprotein 1 Antibodies, IgG and IgM and Cardiolipin Antibodies, IgG and IgM
Container/Tube: Serum gel tube
Specimen: 2 mL (minimum volume: 1 mL) of serum
Transport Temperature: Refrigerated
Note: Label the specimen appropriately (serum for beta-2 glycoprotein 1 antibodies, IgG and IgM and cardiolipin antibodies, IgG and IgM.

Plasma for all Others
Container/Tube: Light blue top (sodium citrate) tubes
Specimen: 6 mL (minimum volume: 3 mL) of sodium citrate platelet poor plasma
Transport Temperature: Frozen (plasma)

Refer to Blue Top Tube (3.2% Sodium Citrate) Collection Instructions

Collection Instructions:
1. Centrifuge specimen in a centrifuge that has been validated to ensure platelet poor plasma.
2. Immediately transfer the plasma to a stoppered, plastic tube and centrifuge again for 10 minutes.
3. Transfer the supernatant plasma into at least 3 screw-capped, plastic vials and send frozen at ≤ -20 °C, if possible.
4. If unable to centrifuge, forward the unprocessed sodium citrate whole blood (minimum volume: 2 mL) to the laboratory promptly at room temperature only.
5. Please complete a "Coagulation Request Form",  list any anticoagulants the patient is receiving, and forward with the specimens.
6. Label the specimens appropriately (citrate plasma).

81240 - Prothrombin Gene Mutation (EAP 30150731)
81241 - Factor V leiden (if appropriate)
85300 - Antithrombin (EAP 30020060)
85303 - Protein C Functional (EAP 30022380)
85307 - APC-R (EAP 30020020)
85302 - Protein C Antigen (if appropriate)
85305 - Protein S (antigenic-total) (if appropriate)
85306 - Protein S (antigenic-free) (if appropriate)
85610 - Prothrombin Time (EAP 30020286)
85613 - dRVVT (if appropriate)
85730 - PTT (EAP 30020288)
86146 - Beta-2 glycoprotein 1 IgG (EAP 30020073)
86146 - Beta-2 glycoprotein 1 IgM (EAP 30020075)
86147 - ACA IgG (EAP 30010040)
86147 - ACA IgM (EAP 30010060)
85597 - Hexagonal phase phospholipid test (if appropriate)
85670 - Thrombin time (if appropriate)
85730x3 - APTT mixing studies (if appropriate)