APL II

Antiphospholipid Antibody Profile II

EPIC Test Procedure Code: LAB2806

Performing Lab:
Saint Luke's Regional Laboratories
Container Type:
Serum gel tube and light blue top (sodium citrate) tubes
Specimen Type:

Serum and sodium citrate plasma are required for this test.

Preferred Volume:
2 mL serum and 6 mL plasma
Minimum Volume:
1 mL serum and 3 mL plasma
Specimen Processing:

1. For serum specimens, label the tube appropriately (serum for beta-2 glycoprotein 1 antibodies, IgG and IgM and cardiolipin antibodies, IgG and IgM.)
2. For plasma specimens, centrifuge specimen in a centrifuge validated to ensure platelet poor plasma. 
3. Immediately transfer the plasma to a stoppered, plastic tube and centrifuge again for 10 minutes. 
4. Transfer the supernatant plasma into 3 screw-capped, plastic vials (each containing 1 mL) and send frozen at ≤ -20 °C, if possible.
5. If unable to centrifuge sodium citrate tube, forward unprocessed sodium citrate whole blood (minimum volume: 3 mL) to the laboratory promptly at room temperature only.
6. Label the specimens appropriately.

Store and Transport:
Serum - refrigerated; Plasma - frozen
Stability:

The unspun specimen must arrive within 2 hours of collection.

CPT Codes:

85610 - Protime APL (EAP 30022490)
85613 - DRVVT APL (EAP 30020442)
85730 - APTT APL (EAP 30022090)
86146 - Beta-2 glycoprotein 1 IgG (EAP 30020073)
86146 - Beta-2 glycoprotein 1 IgM (EAP 30020075)
86147 - ACA IgG (EAP 30010040)
86147 - ACA IgM (EAP 30010060)
85597 - Hexagonal phase phospholipid test (if appropriate)
85670 - Thrombin time (if appropriate)
85730 x 3 - APTT mixing studies (if appropriate)

Test Schedule:
See individual test listings.
Test Includes:
Activated Partial Thromboplastin Time (APTT), Dilute Russell Viper Venom Time (dRVVT), Activated Partial Thromboplastin Time (APTT) Mixing Studies, Hexagonal Phase Phospholipid Test, Beta-2 Glycoprotein 1 Antibodies, IgG and IgM, Prothrombin Time (PT), Cardiolipin Antibodies, IgG and IgM, and Thrombin Time
Reference Ranges:

See individual test listings.

Lab Personnel

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