VWF PF

VWD PL

Saint Luke's Regional Laboratories

Patient's platelet count must be ≥ 100,000 and hematocrit must be ≥ 30% for  PFA-100®). testing.

Two (2)- Light blue-top (sodium citrate) tube(s) - Glass vial is not acceptable

Plasma (sodium citrate) and Whole blood (sodium citrate)

4 mL each, plasma and whole blood; (see specimen collection procedure for "Special Handling")

2 mL each, whole blood and plasma

Refer to Blue Top Tube (3.2% Sodium Citrate) Collection Instructions

*** Special Handling ***

Whole Blood for PFA-100 ®:

1. Do not centrifuge.
2. Forward unprocessed whole blood promptly (must arrive within 2 hours of draw) at room temperature only.
3. Label specimen appropriately (whole blood for PFA-100 ®).

Sodium Citrate Plasma for all Others:

1. Centrifuge at 2,500 rpm for 10 minutes.  Immediately transfer plasma to stoppered, plastic tubes and centrifuge again at 2,500 rpm for 10 minutes.
2. Transfer supernatant plasma into 2 screw-capped, plastic vials (each containing approximately 1 mL) and send frozen at ≤ -20 °C, if possible.
3. If unable to centrifuge, forward unprocessed sodium citrate whole blood (minimum volume: 2 mL) to laboratory promptly at ambient temperature only.
4. Unspun specimen must arrive within 4 hours of draw.
5 Label specimen appropriately (plasma for all others).

Room temperature - whole blood (see "Special Handling"); and
Frozen - plasma (see "Special Handling")

Specimens must arrive within 2 hours of draw.

85240 - Factor VIII Assay (EAP 30020660)
85246 - Von Willebrand Factor (EAP 30023000)
85245 - VWF Activity (EAP 30023010)
85576 - Collagen/ADP (EAP 30022389)
85576 - Collagen/EPI (EAP 30022387)

See individual test listings.

Coagulation Factor VIII (8) Assay (FVIII), Platelet Function Assay (PFA-100®) (PFA 100), von Willebrand Factor Activity (VFW ACT), von Willebrand Factor Antigen (VWF AG)