GI PL INF

Gastrointestinal Pathogen Panel for Infants

EPIC Test Procedure Code: LAB4890

Performing Lab:
Saint Luke's Regional Laboratories
Container Type:
Sterile screw cap cup, Copan Fecal Swab, Cary Blair
Specimen Type:

Stool/Feces

Minimum Volume:
Representative portion of stool. 0.5mL of stool.

Collection Procedure:

1. Each FecalSwab™ kit consists of a sterile peel pouch containing a regular Nylon FLOQSwab™ and a screw cap tube containing 2 mL of Cary-Blair medium. Note: These instructions indicate use of FecalSwab™ after patient has delivered stool specimen in a clean, dry pan or special container. Stool specimen should not contain urine or water.
2. Open the peel pouch. Remove the swab. Do not touch the swab tip. Always hold the shaft applicator above the marked breakpoint.
3. If the patient has a diaper, apply a urine bag to a diapered child. Then wash the child's rectal area using a washcloth with soap and warm water to help decrease the risk of cross contamination.
4. Collect a small amount of stool by inserting all the tip of the flocked swab into the stool sample and rotate it. Note: Bloody, slimy or watery stools should be selected and sampled.
5. Remove and examine to make sure there is fecal material visible on the tip of the swab. If needed, insert again the flocked swab into the stool sample and rotate making sure all the area of the swab tip is in contact with the sample.
6. Transfer the swab into the FecalSwab™ tube and check that the maximum filling line ("MAX.FILL") on the label is not exceeded. Note: If sample collected exceeds maximum fill line, discard the swab and the tube and collect a new specimen using a different FecalSwab™.
7. Holding the swab shaft between thumb and finger, mash and mix the stool specimen against the side of the tube to evenly disperse and suspend the specimen in the medium.
8. Hold the tube away from your face. Holding the end of the swab shaft, bend it at a 180 degree angle to break at the marked breakpoint.
9. If needed, gently twist the shaft between thumb and forefinger to completely remove it. Discard the broken upper part of the swab shaft and tighten the cap.
10. Shake the vial until the sample appears homogenous.
11. Write patient's name and demographics on the tube or apply a label and send the sample to the laboratory.

Specimen Processing:

Specimen must arrive within 96 hours of collection.

Store and Transport:
Fecal Swab - Room temperature
Stability:

Ambient (preferred) - 4 days
Refrigerated - 4 days

Unacceptable Condition:

Swabs (non-Fecal Swab) are not acceptable for testing.

CPT Codes:

0097U - Gastrointestinal Pathogen Inf Pl (EAP 30660345)

Test Schedule:
Monday through Sunday
Method:
Multiplexed DNA probe test
Test Includes:
Targets detected: Campylobacter, Plesiomonas shigelloides, Salmonella, Vibrio, Vibrio cholera, Yersinia enterocolitica, Enteroaggregative E. coli (EAEC), Enteropathogenic E. coli (EPEC), Enterotoxigenic E. coli (ETEC) It/st, Shiga-like toxin producing E. coli (STEC) stx1/stx2, E. coli 0157, Shigella/Enteroinvasive E. coli (EIEC), Cryptosporidium, Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia, Adenovirus F 40/41, Astrovirus, Norovirus GI/GII, Rotavirus A, Sapovirus I, II, IV, and V
Reference Ranges:

Not detected

Lab Personnel

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