Monkeypox

Monkeypox (Orthopoxvirus), DNA, PCR

EPIC Test Procedure Code: LAB6697

Performing Lab:
Referral Laboratory
Container Type:
Universal Transport Media (UTM) or Viral Transport Media (VTM)
Specimen Type:

Lesion swab

Preferred Volume:
One swab in UTM or VTM
Minimum Volume:
One swab in UTM or VTM
Collection Procedure:

Vigorously swab or brush the base of the lesion with a sterile dry polyester, rayon or Dacron swab. Insert the swab into the tube containing UTM or VTM. Carefully break the swab at the scoreline and tightly close the sample. Some UTM kits may contain two swabs; however, only one swab needs to be collected and submitted for testing. If multiple lesions with differing appearances are present, consider submitting an additional UTM/VTM collection, as described above, for each lesion. 

Any viral transport medium manufactured by KANG JIAN  is unacceptable!

Renji Medical tube is unacceptable.

Covid swab/media is unacceptable.

Store and Transport:
Refrigerate
Stability:

Refrigerated: 7 days

Frozen: 30 days

Unacceptable Condition:

Improperly submitted specimens, including grossly leaking samples; swabs submitted in non-validated transport media, including 0.9% saline or liquid Amies (E-Swabs); swab specimens submitted in aerobic or anaerobic transport devices

Limitations:
A false negative result may occur if a sample is improperly collected, transported or handled. False negative results may occur if the quantity of viral DNA present in the sample is below the limit of detection for the assay. An inconclusive result may be returned if the lesion is not adequately sampled.
CPT Codes:

87593 - Monkeypox (Orthopoxvirus), PCR (EAP 30621200)

Turnaround Time:
3-5 days
Method:
Polymerase chain reaction (PCR)
Reference Ranges:
Clinical Significance:
Vesicular specimens collected from persons infected with a non-variola Orthopoxvirus (such as vaccinia, monkeypox, or cowpox) are expected to produce a positive result with this assay. Although this assay does not differentiate vaccinia or monkeypox virus from cowpox, camelpox, ectomelia or gerbilpox virus, a positive result with this assay in the United States is most likely due to monkeypox virus or vaccinia virus; however, potential exposure to other Orthopoxviruses should be considered.
Notes:

If monkeypox is suspected, do not submit specimens for any Viral Cultures. 

Reflex Test :

Lab Personnel

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